Fig. 2

SCMECs UTX KO promoted the migration and neural differentiation of NSCs after SCI. A Immunofluorescence analysis of the migration of endogenous NSCs on the 3rd and 7th day after SCI in UTX KO and NC mice. Scale bar, 100 μm. B Statistical analysis of the distance from tdTomato⁺ cells to the center of injury (mm) in figure A, n = 6 per group. C Immunofluorescence analysis of spatial distribution of NeuN⁺ cells (neuron marker) and GFAP⁺ cells (astrocyte marker) in UTX KO and NC mice in the sham group and 14 days after SCI. Scale bar, 40 μm. D Statistical analysis of the density of NeuN⁺ cells in the injured area after 14 days of SCI (mm²) in figure C, n = 6 per group. E Nestin (NSC marker) and SOX2 (NSC marker) immunofluorescent identification of primary lsolated neurosphere formed by NSCs aggregation. Scale bar, 20 μm. F Immunofluorescent analysis of the neural differentiation and astrocytic differentiation of NSCs in vitro intervened by UTX KO SCMECs supernatant (UTX KO group) and NC SCMECs supernatant (NC group). Scale bar, 40 μm. G, H Statistical analysis of Tuj-1⁺ cells and GFAP⁺ cells to all cells in each group in figure F, n = 10 per group. ^nsP > 0.05, *P < 0.05, **P < 0.01, compared with corresponding control group