Fig. 6
From: Impaired bisecting GlcNAc reprogrammed M1 polarization of macrophage
The correlation of NF-κB, Lgals3bp, and MGAT3. a GO analysis of differentially expressed proteins in M0-M1 polarization of RAW264.7 cells. b, c Expression of p65, p-p65, IкBα and p-IкBα in RAW264.7 cells stimulated by LPS and IFN-γ (b) or RAW/shM1 cells (c) by western blotting. d Interaction between Lgals3bp and Traf6 in RAW/shM1 cells by Co-IP. e MGAT3 mRNA level in RAW264.7 cells stimulated by LPS and IFN-γ. f PROMO analysis to predict the transcription factors that bind to the MGAT3 promoter. g Dual-luciferase reporter assays in HEK 293 T cells transfected with p65 and the MGAT3 promoter sequence