Fig. 5

The function of bisecting GlcNAc on Lgals3bp. a Bisecting GlcNAc modification on Lgals3bp in RAW/shM1 cells by IP. b The Lgals3bp expression after CHX (150 µg/mL) treatment for indicated times was detected by western blotting. c The ubiquitination of Lgals3bp after MG132 (5 μM) treatment for 12 h in RAW264.7/shM1 cells was detected by Co-IP/western blotting. d Interaction of Lamp2 and Lgals3bp in RAW264.7/shM1 cells was detected by Co-IP/western blotting. e Flag-tagged wild type (WT/LG) or deglycosyl mutant at position 69 (N69A/LG) of Lgals3bp was transfected into HEK 293 T cells. f The expression of WT or N69A mutant of Lgals3bp was detected by western blotting after treatment with CHX (150 µg/mL) for the indicated times. g The ubiquitination of WT or N69A mutant of Lgals3bp was detected by Co-IP/western blotting after treatment with MG132 (5 μM) for 12 h in HEK 293 T cells. h The interaction between Lamp2 and WT or N69A mutant of Lgals3bp in HEK 293 T cells was detected by Co-IP/western blotting. i RAW264.7 cells were treated with CHX and MG132 or Chl for 12 h, then Lgals3bp expression were detected by western blotting