Fig. 3
From: Impaired bisecting GlcNAc reprogrammed M1 polarization of macrophage
Lgals3bp identified as a bisecting GlcNAc modified glycoprotein. a Work flow of proteomics and glycoproteomics analysis by LC–MS/MS. b Volcano map of differentially expressed protein in M0-M1 polarization of RAW264.7 cells. c, d Differentially expressed glycoproteins in M0-M1 polarization of RAW264.7 cells by Venn diagram (c) and heatmap (d). e Representative MS/MS spectrum of ALGYEN#ATQALGR of Lgals3bp with bisecting GlcNAc in RAW264.7 cells. f Lgals3bp expression or its bisecting GlcNAc modification, and whole cellular level of bisecting GlcANc modification in M0-M1 polarization of RAW264.7 cells by western blotting or immunoprecipitation (IP). WCL, whole cell lysate