Fig. 2
From: Impaired bisecting GlcNAc reprogrammed M1 polarization of macrophage
Effects of bisecting GlcNAc on M0-M1 polarization. MGAT3 was silenced in RAW264.7 cells (RAW/shM1/2). a Bisecting GlcNAc level and MGAT3 expression in RAW/shM1 cells by lectin/western blotting. b MGAT3 mRNA level in RAW/shM1 cells by qRT-PCR. c&d Bisecting GlcNAc level (c) and CD86 expression (d) in RAW/shM1 cells by FACS. e CD86 expression in RAW/shM1 cells by immunofluorescence. f Balb/c mice were subcutaneously injected with a mixture of CT26 cells and either RAW264.7 or RAW/shM1 cells. g Tumor weight. h Purified macrophages from tumor tissue stained by anti-F4/80, lectin PHA-E or anti-CD86