Fig. 7
From: A time window for rescuing dying retinal ganglion cells
Mitochondrial impairment cannot be inhibited by caspase inhibitor. prRGCs were treated with zVAD (20 μm, 4 h) or EtOH (5%, vol/vol, 3 h), or pretreated with zVAD for 1 h and then co-treated with EtOH for another 3 h. A Percentage of cells with fragmented mitochondria. B Quantified mitochondrial membrane potential by the fluorescence intensity of TMRM staining in living prRGCs. C and D Representative ultrastructure images of RGC mitochondria in soma and neurite. Scale bar: 1 μm. E-H Quantification of mitochondrial area, length, perimeter, and cristae number per mitochondria. At least 300 mitochondria are quantified in each group. Data are presented as the mean ± SEM. ****p < 0.0001. EtOH: ethanol