Fig. 1
From: A time window for rescuing dying retinal ganglion cells
Dynamics of PS exposure in EtOH treated prRGCs. Time-lapse microscopy analysis (6 frames per hour) of the spatial and temporal pattern of PS externalization in rat prRGCs. Cells were imaged with annexin A5-FITC present in the culture medium for the duration of the experiment. Negative control (NC) group showed that staining and imaging were not cytotoxic (A). B Time-lapse images showing the progressive movement of PS exposure along neurites to soma after EtOH treatment. White arrow points where annexin A5-FITC staining was first observed in neurites. C Time-lapse images showing the progressive movement of PS exposure which started from the soma. White arrow indicated the starting point of PS exposure. D The percentage of prRGCs in which PS exposure started from neurite or soma. 66 cells were imaged in total. EtOH: ethanol. Scale bar: 50 μm. Time: hr.:min