Fig. 2

A 3D structure of AD-GroEL (PDB: 1DER, residues from 191 to 335). The numbering of amino acids corresponds to the numbering in the structure of a full-length protein. The protein is colored from N- to C- terminus according to the color scale at the bottom of the Figure. Thirteen glycines are denoted as orange or dark grey. The six glycines selected as cleavage points are colored in orange and are labeled by their residue number. The other glycines are shown in dark grey. B Probability of intrinsic disorder of the AD-GroEL circular sequence calculated by the PONDR® VLXT program. The circles denote the glycines at the site of the designed breaks in the amino acid sequence