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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: IL13Rα2 as a crucial receptor for Chi3l1 in osteoclast differentiation and bone resorption through the MAPK/AKT pathway

Fig. 4

IL13Rα2 silencing and rIL13Rα2 protein inhibit osteoclast differentiation. A After silencing the IL13Rα2 expression in BMMs with IL13Rα2 siRNA, M-CSF and RANKL were applied to treat BMMs for 5 days to induce osteoclast differentiation. TRAP staining revealed that the formation of mature multinucleated osteoclasts was inhibited by IL13Rα2 siRNA. B PCR analysis also revealed that the mRNA expression of the osteoclast differentiation-related genes was significantly down-regulated by IL13Rα2 siRNA. C M-CSF and RANKL were applied to treat BMMs for 5 days to induce osteoclast differentiation with 500 ng/ml or 1000 ng/ml rIL13Rα2 protein. TRAP staining discovered that mature multinucleated osteoclasts were dose-dependently attenuated by rIL13Rα2 protein. D PCR results demonstrated that the mRNA expression of the osteoclast differentiation-related genes was dramatically suppressed by 1000 ng/ml rIL13Rα2 protein. **P < 0.01. Data are presented as the means ± SD

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Cell Communication and Signaling

ISSN: 1478-811X