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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: IL13Rα2 as a crucial receptor for Chi3l1 in osteoclast differentiation and bone resorption through the MAPK/AKT pathway

Fig. 2

Chi3l1 regulates the RANKL-induced MAPK/AKT pathway activation. A BMMs were induced with RANKL, 500 ng/ml Chi3l1 recombinant protein, or RANKL+ 500 ng/ml Chi3l1 recombinant protein for 0, 10, 20, 30, and 60 mins. Then the signaling pathway phosphorylation was analyzed by western blot. B Quantitative densitometric analysis was performed to normalize p-AKT, p-ERK, p-P38, p-JNK, and p-P65 expressions from (A). C After silencing Chi3l1 expression in BMMs using Chi3l1 siRNA for 48 h, RANKL was used for stimulating BMMs for 0, 10, 20, 30, and 60 mins. The signaling pathway activation was analyzed by western blot. D Quantitative densitometric analysis was conducted to normalize the aforementioned expressions from (C). **P < 0.01. Data are presented as the means ± SD

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Cell Communication and Signaling

ISSN: 1478-811X