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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: IL13Rα2 as a crucial receptor for Chi3l1 in osteoclast differentiation and bone resorption through the MAPK/AKT pathway

Fig. 1

Chi3l1 recombinant protein promotes but Chi3l1-siRNA inhibits osteoclast differentiation of BMMs. A M-CSF, RANKL, and various concentrations of Chi3l1 recombinant protein (0, 125, 250, 500, 1000 ng/ml) were applied to treat BMMs for 5 days. TRAP staining showed that recombinant Chi3l1 protein dose-dependently induced mature multinucleated osteoclast formation. B PCR analysis showed that the mRNA expression level of osteoclast differentiation-related genes was significantly upregulated by 500 ng/ml Chi3l1 recombinant protein. C After silencing Chi3l1 with Chi3l1 siRNA, M-CSF and RANKL were applied to treat BMMs for 5 days to induce osteoclast differentiation. TRAP staining showed that mature multinucleated osteoclasts were significantly inhibited by Chi3l1 siRNA. D PCR analysis also revealed that the mRNA expression of the osteoclast differentiation-related genes was significantly down-regulated by Chi3l1 siRNA. **P < 0.01. Data are presented as the means ± SD

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Cell Communication and Signaling

ISSN: 1478-811X