Fig. 6

SLC22A17 deposition in the hippocampal area and AP-UA axis regulated by PA. A Representative IHC sections of SLC22A17 deposition and expression compared in the hippocampal areas in three groups (3 mice each group). Red arrowheads indicate SLC22A17 immunoreactive areas. Scale bar = 500 μm. B Quantification of iron, LD, Pi, AP and UA levels indicated that PA not only counteracted the HFe-induced increase in levels of LBP, LCN2 and phosphate acid, but also increased levels of UA and AP in serum from the mice (above 8 mice each group). One-way ANOVA with post-hoc LSD test was used for significance. C Quantification of AP activity indicated that PA significantly increased AP activity in the colon and duodenum of mice (above 5 mice each group).One-way ANOVA with post-hoc LSD test was used for significance. D Lifespan extension for C.elegans VH254 fed with E. coli OP50 after adding 0.5 mM UA compared to controls (0 mM UA) from the L4 developmental stage maintained at 15 °C (120 worms per group).One-way ANOVA with post-hoc LSD test was used for significance. E mRNA expression of UA transport genes of worms from the p-tau control and UA-treated group (600 worms per group).One-way ANOVA with post-hoc LSD test was used for significance. All data are means ± SEM. Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001, ns: no significant difference