Fig. 4

Effects of PA on cortical tissue under the HFe diet. A Western blotting showed the effects of PA on the expression levels of proteins involved in iron accumulation, cell death, AD risk makers and Pi transporters in the cortical tissue of the mice (3 mice each group). B Quantification of iron, LD, Pi, AP and UA levels indicated that PA counteracted the HFe-induced increase in levels of iron in cortical tissue but had no effect on cortical LD and Pi levels; in contrast, PA significantly increased AP and UA levels in cortical tissue compared to HFe-fed mice (above 5 mice each group).One-way ANOVA with post-hoc LSD test was used for significance. C Representative fluorescence sections of p-tau deposition and expression in cortical tissue in three groups by immunofluorescence (3 mice each group). Scale bar = 100 μm or 200 μm. All data are means ± SEM. Statistical significance vs. with the vehicle-only treatment group. *p < 0.05, **p < 0.01, ***p < 0.001, ns: no significant difference