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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Extracellular succinate derived from ectopic milieu drives adhesion and implantation growth of ectopic endometrial stromal cells via the SUCNR1 signal in endometriosis

Fig. 4

The expression of SUCNR1 in cells from Ectopic milieu. A SUCNR1 expression in female tissues from healthy human tissues (data are available from https://www.proteinatlas.org/). B-D The level of SUCNR1+ macrophages and peritoneal mesothelial cells (PMC) in peritoneal fluid from EMs patients was elevated and correlated with EMs stage. E The staining macroscopic observation of peritoneal mesothelial cells (PMC) in peritoneal fluid or intraperitoneal lavage (modified papanicolaou staining, 40X). F-G FCM-based MFI assay and qT-PCR analysis designed to measure SUCNR1 expressions, and result showed primary ectopic stromal cell (hESC.D) expressed higher SUCNR1, compared with that of primary normal stromal cell (hESC.N) and human stromal cell line (hESC.N). H-I FCM and qT-PCR assays showed the SUCNR1 expression of primary normal ESC stimulated with succiante with different concentrations. The optimal concentration of succinate is 2.5 mM. Significance was assessed either with t test or one-way ANOVA followed by Tukey’s or Holm-Sidak’s post-test. *p < 0.05, **p < 0.01, ***p < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X