Skip to main content
Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Liquid plasma promotes angiogenesis through upregulation of endothelial nitric oxide synthase-induced extracellular matrix metabolism: potential applications of liquid plasma for vascular injuries

Fig. 6

LP induces endothelial cell migration and angiogenesis in vivo. Analysis of the survival rates. No statistically significant difference was observed in the survival rate between the LP-treated group, the non-treated group (N = 10, each group), and the vascular endothelial growth factor (VEGF)-treated group (positive control). B The Matrigel plug assay. Representative photomicrographs showing angiogenesis in response to the injected Matrigel. Capillary formation towards the Matrigel was thickened in the LP-treated group compared to that in the control group; scale bar = 20 μm. (C) H and E staining of Matrigel plug sections from control and LP-treated mice; scale Bar = 200 μm. D Image shows the morphology of Matrigel plugs harvested from LP-treated and control mice; scale bar = 20 mm. E Hemoglobin content is shown in graphs (N = 10 per group). Data are presented as mean ± SEM. **P < 0.01, **P < 0.01, and ***P < 0.001 Student t test. F, G Immunofluorescence analysis of endothelial cell marker CD31 (red) and NO signaling pathway marker p-eNOS. p-AMPK levels in the sections of the Matrigel plugs derived from control and LP-treated mice are shownl nuclei are labeled using DAPI (blue); scale bar = 100 μm. Quantification of CD31-positive cells (10 fields/group), p-eNOS(S1177), p-AMPK (Thr172). ***P < 0.001

Back to article page

Cell Communication and Signaling

ISSN: 1478-811X