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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Liquid plasma promotes angiogenesis through upregulation of endothelial nitric oxide synthase-induced extracellular matrix metabolism: potential applications of liquid plasma for vascular injuries

Fig. 4

NOS inhibitors (L-NMMA, 500 μM) can selectively regulate eNOS expression, angiogenesis, and migration. A The expression of total eNOS and its phosphorylation were analyzed by Western blot. Phosphorylation of eNOS was upregulated after treatment with LP and was significantly attenuated by L-NMMA. The band intensities were measured and are represented as a graph. ***P < 0.001. B Flow cytometry using DAF-FM probes in cells treated with L- NMMA. The bar graph presents the mean ± standard deviation of three independent experiments. ***P < 0.001. C Angiogenic activity in LP and L-NMMA-treated HUVECs assessed using tube formation assay; scale bar = 1000 μM. Bar graph showing quantification of tube formation. N = 5, ***P < 0.001. Effect of LP treatment on the migratory potential of LP- and L-NMMA-treated HUVECs using (D) wound migration assay. Bar graph showing quantification of cell migration. ***P < 0.001, NS = not significant; scale bar = 1000 μM. E Zymogram assay. The activation of MMP-2 by LP was significantly attenuated by the L-NMMA treatment. The band intensities were measured and are represented as a graph. ***P < 0.001, NS = not significant, (F) Expression of VE-cadherin was decreased after treatment with LP and was significantly attenuated by L-NMMA; scale bar = 100 μm

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Cell Communication and Signaling

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