Fig. 7

Tet2 chemical inhibitors promote liver regeneration dependent on macrophages. Mice were intraperitoneally injected with BC339 (20 mg/kg) every other day for 1 week. CL was used to deplete macrophages before and after PHx. We performed a modified 70% partial hepatectomy (PHx) model. Whole liver tissue was harvested 48 h after PHx. A Kaplan–Meier analysis was used to determine the survival rate of mice after PHx with the depletion of macrophages. We observed the survival rate of mice every 12 h and up until 72 h. B Mice were euthanized at 48 h after PHx. The liver-to-body weight ratio. Liver to body weight ratio = liver weight/body weight (g/g). C, D ALT (C) and AST (D) serum levels were measured 48 h after PHx. E The Tert, Lgr5, and Sox9 mRNA expression levels were examined using qPCR analysis. The gene was normalized to GAPDH mRNA levels in each sample. F, G Co-staining of Ki67 + and Hnf4α + (F) and the ratios of Ki67 + Hnf4α + hepatocytes are shown (G). (A, n = 10, B to D n = 6, E to G, n = 3, *p < 0.05.)