Fig. 6

Tet2 inhibits macrophages secreting IL-6 and decreases liver regeneration Mice were intraperitoneally injected with Bobcat339 (BC339; 20 mg/kg) every other day for 1 week, and PHx was performed. Mice were euthanized at 48 h after PHx. Whole liver tissue was harvested 48 h after PHx. A Liver-to-body weight ratio at 48 h. Liver to body weight ratio = liver weight/body weight (g/g). B, C, D Serum levels of ALT (B), AST (C), and IL-6 (D) were measured 48 h after PHx. E, F The mRNA expression levels of IL-6 (E), Sox9, Tert, and Lgr5 (F) were examined using qPCR analysis (The genes were normalized to GAPDH mRNA levels in each sample. Sox9: SRY-box transcription factor 9, Tert: telomerase reverse transcriptase, Lgr5: leucine-rich repeat-containing G protein-coupled receptor 5). G, H Co-staining of Ki67 + and Hnf4α + (G) and the ratios of Ki67 + Hnf4α + hepatocytes (H). Ki67: proliferation marker (Ki-67), Hnf4α: hepatocyte nuclear factor 4 alpha. A to D, n = 6, E to H, n = 3, *p < 0.05.)