Fig. 3
From: Ten-eleven translocation-2-mediated macrophage activation promotes liver regeneration
Tet2 negatively regulates liver regeneration after PHx. We analyzed the change in epigenetic factors after PHx using the RNA-seq (GSE158864) and scRNA-Seq databases (GSE124395) published in the Gene Expression Omnibus. Correlation analysis of Tet2 was downloaded from GEPIA. Flow cytometry was used to verify the results. A Heatmap of 23 vital epigenetic modification enzymes 0 and 48 h after PHx. B Expression t-SNE maps of Tet1, Tet2, and Tet3 in healthy human livers. The color bar indicates log2 normalized expression, and the violin plot of Tet2 expression for all clusters is shown. C Correlation analysis showed the Tet2 level with ITGAM (macrophage marker gene) and ALB (hepatocyte marker gene) in normal human liver cells. D, E, F Flow cytometry showing the percentage of Tet2+ macrophages (Tet2+F4/80−CD45+CD11b+F4/80+), Tet2+NK cells (Tet2+F4/80−CD45+NK1.1+CD3ε−), and Tet2+T cells (Tet2+ F4/80−CD45+ CD3ε.+) in each immune cell type (D to F, n = 6. *p < 0.05)