Fig. 2
From: Ten-eleven translocation-2-mediated macrophage activation promotes liver regeneration
Macrophages increased significantly after PHx. Mice were anesthetized with isoflurane, and a PHx model was constructed. Mice were euthanized, and immune cells were harvested at 0, 24, and 48 h after PHx using a modification of the collagenase perfusion method. Flow cytometry was used to detect the number of each immune cell. The gating strategy is shown in Fig. S2A. A Flow cytometry showed the percentage of total macrophages (CD45+CD11b+F4/80+) in total immune cells (CD45+). B Flow cytometry showed the percentage of Kupffer cells (KCs; CD45+CD11b+F4/80+CLEC4F+) and monocyte-derived macrophages (MoMFs; CD45+CD11b+F4/80+CLEC4F−) in total macrophages. C, D Flow cytometry showed the percentage of B (F4/80−CD45+CD20+), T (F4/80−CD45+ CD3ε+), and NK1.1 cells (F4/80−CD45+NK1.1+ CD3ε−) in total immune cells. E, F Immunofluorescent staining of F4/80+ macrophages. (A to G, n = 6. *p < 0.05.)