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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Paxillin participates in the sphingosylphosphorylcholine-induced abnormal contraction of vascular smooth muscle by regulating Rho-kinase activation

Fig. 5

Paxillin knockdown inhibits SPC-induced Rho-kinase activation but not SPC-induced Fyn activation. a, Fyn activity in paxillin knockdown human CASMCs in the absence and presence of SPC (30 µM, 5 min), using immunoprecipitation and Western blot. We used Fyn antibody to obtain Fyn and then detected Y420 phosphorylation of Fyn (pY420Fyn) with the Src Y416 phosphorylated antibody that cross-reacts with pY420Fyn. b, Statistical analysis of Fyn activation (Ratio of pY420Fyn to total Fyn after Fyn immunoprecipitation) in (a). n = 3, *p<0.05, ns: no significant. c and d, The phosphorylation of MYPT1 at site T853 was analyzed by Western blot in paxillin knockdown human CASMCs (c) and paxillin SMKO mice (d). e and f, Statistical analysis of Rho-kinase activation (Ratio of phosphorylated MYPT1 at site T853 to total MYPT1) in paxillin knockdown human CASMCs (e,n = 3) and paxillin SMKO mice (f,n = 4). *p<0.05. Cont: control mice; PXN KO: paxillin SMKO mice

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Cell Communication and Signaling

ISSN: 1478-811X