Fig. 8
From: Copper nanoparticles and silver nanoparticles impair lymphangiogenesis in zebrafish
CuNPs stress induced hypermethylation of E2F7/8 binding sites on CCBE1 promoter in HUVECs. A Methylations of E2F7/8 binding sites on CCBE1 and FLT4 promoters in the control, AgNPs- and CuNPs-stressed HUVECs and the corresponding NAC treated HUVECs. B Luciferase activities of human CCBE1 and FLT4 promoters in the control, AgNPs- and CuNPs-stressed HEK293T cells, respectively. C Binding enrichment of proteins E2F7 (C1) and E2F8 (C2) for human CCBE1 and FLT4 promoters as revealed by chromatin immunoprecipitation assays (ChIP) in the control, AgNPs- and CuNPs-stressed HUVECs. Data are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. NS, not significant