Fig. 7
From: Copper nanoparticles and silver nanoparticles impair lymphangiogenesis in zebrafish
CuNPs rather than AgNPs stress induced ROS stresses in HUVECs. A Reactive oxygen species levels in the control, AgNPs- and CuNPs-stressed HUVECs (A1) by DCFH-DA staining, and quantitative analysis of ROS level in cells from the three groups (A2). B Protein levels of HSF1 in the control, AgNPs- and CuNPs-stressed HUVECs (B1); protein levels of ATF4, CALNEXIN and EIF2A-pS51 in the control, AgNPs- and CuNPs-stressed HUVECs and the corresponding NAC treated HUVECs (B3), and quantitative analysis of protein level in cells of the five groups respectively (B2, B4). Data are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. NS, not significant