Fig. 5
From: Copper nanoparticles and silver nanoparticles impair lymphangiogenesis in zebrafish
CuNPs rather than AgNPs stress induced ROS stresses in zebrafish embryos. A Reactive oxygen species levels in the control (A1), AgNPs- (A3) and CuNPs-stressed embryos (A5) and in the corresponding ROS scavenger NAC co-treated individuals (A2, A4, A6) by DCFH-DA staining. B RT-PCR expression analysis of genes perk, ire1a, bip in the control, AgNPs- and CuNPs-stressed embryos or larvae and in the corresponding NAC co-treated individuals (B1 − B3). C Protein levels of Atf4, Hsf1, Calnexin and Eif2a-pS51 in the control, AgNPs- and CuNPs-stressed embryos or larvae and in the corresponding NAC co-treated individuals (C1, C3, C5), and quantitative analysis of protein level in embryos or larvae from the five groups respectively (C2, C4, C6). D B-C legend. Data are mean ± SD. A1 − A6, lateral view, anterior to the left, and dorsal to the up. *P < 0.05, **P < 0.01, ***P < 0.001. NS, not significant