Fig. 4
From: Copper nanoparticles and silver nanoparticles impair lymphangiogenesis in zebrafish
CuNPs stress induced hypermethylation of E2f7/8 binding sites on ccbe1 promoter. A Methylations of CpG in E2F7/8 binding sites on ccbe1 and flt4 promoters of zebrafish embryos from the control, AgNPs- and CuNPs-stressed groups at 32 hpf. B Luciferase activities of zebrafish ccbe1 or flt4 promoter in the control, AgNPs- and CuNPs-stressed HEK293T cells, respectively. C The representative embryos injected with plasmids of ccbe1 or flt4 promoter-driven GFP from the control, AgNPs- and CuNPs-stressed groups at 52 hpf (C1 − C6), and quantitative analysis of fluorescence in nerves and lymphatic vessels in the representative embryos (C7 − C8). Data are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. NS, not significant. Scale bars, 250 μm (C1 − C6)