Fig. 3
From: Copper nanoparticles and silver nanoparticles impair lymphangiogenesis in zebrafish
Salvage effect of ccbe1/e2f7/e2f8 mRNA on lymphangiogenesis defects in the control, AgNPs- and CuNPs-stressed zebrafish larvae. A Lateral images of lymphatics vessels of the control (A1), AgNPs-stressed (A4), CuNPs-stressed (A7), control injected with ccbe1 mRNA (A2), AgNPs group injected with ccbe1 mRNA (A5), CuNPs group injected with ccbe1 mRNA (A8), control injected with e2f7/8 mRNA (A3), AgNPs group injected with e2f7/8 mRNA (A6), CuNPs group injected with e2f7/8 mRNA (A9) Tg(fli1a:GFP; flk1:mCherry) zebrafish larvae at 120 hpf; blue arrows, flk1 mCherry positive and fli1a GFP negative in lymphatics vessels (thoracic duct), and TD formation was quantitatively analyzed by measuring its presence from segments of somite 5 to somite 15 (A10). Data are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. NS, not significant. Scale bars, 100 μm (A1 − A9)