Fig. 2
From: Copper nanoparticles and silver nanoparticles impair lymphangiogenesis in zebrafish
AgNPs rather than CuNPs stress impaired the expression of transcription factors E2f7 and E2f8. A RT-PCR expression analysis of genes e2f7 and e2f8 in the control, AgNPs- and CuNPs-stressed embryos or larvae at 32 hpf, 52 hpf, and 120 hpf, respectively. B WISH analysis of gene ccbe1 in the AgNPs-stressed embryos, CuNPs-stressed embryos, AgNPs- and CuNPs-stressed embryos with ectopic expression of e2f7 and e2f8 at 32 hpf (B1 − B4), and quantitative analysis of WISH data for gene ccbe1 in the four groups (B5). Data are mean ± SD. B1 − B4, lateral view, anterior to the left, and dorsal to the up. *P < 0.05, **P < 0.01, ***P < 0.001. NS, not significant. Scale bars, 500 μm (B1 − B4)