Fig. 1
From: Copper nanoparticles and silver nanoparticles impair lymphangiogenesis in zebrafish
CuNPs and AgNPs stress impaired lymphangiogenesis via down-regulating ccbe1 expression. A Lateral images of lymphatics vessels of the control (A1), AgNPs-stressed (A2), and CuNPs-stressed (A3) Tg(fli1a:GFP; flk1:mCherry) zebrafish larvae at 120 hpf; blue arrows, flk1 mCherry positive and fli1a GFP negative in lymphatics vessels (thoracic duct), and TD formation was quantitatively analyzed by measuring its presence from segments of somite 5 to somite 15 (A4). B RT-PCR expression analysis of lymphangiogenesis-related genes ccbe1, vegfc, and flt4 in the control, AgNPs- and CuNPs-stressed embryos or larvae at 32 hpf, 52 hpf, and 120 hpf, respectively. C WISH analysis of gene ccbe1 in the control, AgNPs- and CuNPs-stressed embryos at 32 hpf (C1 − C3), and quantitative analysis of WISH data for gene ccbe1 in the three groups (C4). Data are mean ± SD. C1 − C3, lateral view, anterior to the left, and dorsal to the up. *P < 0.05, **P < 0.01, ***P < 0.001. NS, not significant. Scale bars, 100 μm (A1 − A3) and 500 μm (C1 − C3)