Fig. 2

rTGFβ1 inhibited ERK1/2 signaling and immune reaction of RS218 infected hBMECs relying on inducted noncanonical HH signaling. A Detecting expression alterations of IL-6, MIP-2, and E-selectin in hBMECs with qPCR or Western blot. The cells were infected with RS218 with or without rTGFβ1 pre-treatment (at 50 ng/mL). **p < 0.01. The qPCR assays were performed in triplicates, and results are presented as mean ± SEM. B qPCR detecting IL-6, MIP-2, and E-selectin transcription upon RS218 infection in wild-type hBMECs or Gli-KO hBMECs. The wild-type hBMECs were pretreated with rTGFβ1 (at 50 ng/mL) or together with GANT61 (at 10 μM). The Gli-KO hBMECs were pretreated with rTGFβ1 (at 50 ng/mL). **p < 0.01. The qPCR assays were performed in triplicates, and results are presented as mean ± SEM. C Western blot detecting phosphorylation of p65, JNK, ERK1/2, and p38 in RS218 infected hBMECs. The cells were pretreated with or without rTGFβ1 at 50 ng/mL. D Western blot detecting expression of E-selectin and ERK1/2 phosphorylation in RS218 infected hBMECs. The cells were pretreated with rTGFβ1 (at 50 ng/mL) or GANT61 (at 10 μM). E Detecting expression alterations of IL-6, MIP-2, and E-selectin in hBMECs with qPCR or Western blot. The cells were infected with RS218 with or without U0126 treatment (at 5 μM). **p < 0.01. The qPCR assays were performed in triplicates, and results are presented as mean ± SEM