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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Synergism of non-thermal plasma and low concentration RSL3 triggers ferroptosis via promoting xCT lysosomal degradation through ROS/AMPK/mTOR axis in lung cancer cells

Fig. 5

NTP + RSL3 treatment promotes xCT lysosomal degradation via ROS/AMPK/mTOR axis. A Transcription level of xCT. B Effect of NH4Cl (20 mM) or MG132 (5 μM) on the expression of xCT protein. C The protein level of mTORC1 pathway and phosphorylation of AMPK after NTP + RSL3 treatment. D Effect of L-leucine (20 mM) on the protein expression of xCT. E Effect of rapamycin (100 μM) on the protein expression of xCT. F Effect of NAC (5 mM) on the expression of mTORC1, AMPK phosphorylation and xCT expression. G The interference effect of siRNA on AMPK expression in Calu-1 cells. H The levels of mTORC1 phosphorylation and xCT expression after NTP + RSL3 with siRNA treatment in Calu-1 cells. I Schematic overview of mechanism underlying the ferroptosis induction by NTP + RSL3 via ROS/AMPK/mTOR axis promoting xCT lysosomal degradation

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Cell Communication and Signaling

ISSN: 1478-811X