Fig. 5

NETs induce procoagulant activity in the AIS process. Control neutrophils were incubated with PMPs from healthy controls (a) and AIS patients (b) and stained with TF (red) and MPO (green) and observed by confocal microscopy. The expression of TF on neutrophils healthy controls (n = 2) and AIS patients (n = 2) detected by western blots. d Levels of TAT complexes in plasma from healthy subjects (n = 45) and AIS patients (n = 45) were detected by ELISA. e Control plasma was incubated with isolated NETs (0.5 µg DNA/ml) and treated with an anti-TF antibody and DNase I in inhibition assays. f Control plasma was incubated PLTs treated with NETs and in the presence of DNase I. g-i PLTs were stained with CD41 (green) and fibrin (red) in each group. Statistics, Mann-Whitney test (d) and ordinary ANOVA (e, f). Data are presented as the mean ± SD. *P < 0.05, **P < 0.01 and ****P < 0.0001