Fig. 6
IL-4 induces an increase in SMYD3-mediated H3K4me3 modification of IGF2 expressed in primary human nasal epithelial cells to promote the differentiation of naïve CD4 + cells into Th2 cells. A-B IGF2 stimulation induces the differentiation of naïve CD4 + T cells into Th2 cells, as determined by FACS. C The concentrations of a range of secreted classical cytokines associated with Th2 and Th1 cells (IL-4, IL-13, IL-5, IFN-γ and IL-2) were measured with ELISA kits (mean ± s.d. n = 6). Statistical significance,Mann–Whitney U test;*** p < 10.−3;* p < 0.05; ns, not significant (p > 0.05). D IGF-2 derived from nasal epithelial cells induces the differentiation of naïve CD4 + T cells into Th2 cells, as determined by FACS (n = 6 donors)