Fig. 5

IL-4 stimulation increased the H3K4me3 level in IGF2 and increased IGF2 expression. A Integrated Genomics Viewer (IGV) visualization of the H3K4me3 enrichment status across the IGF2 gene, revealing that IL-4 promoted high H3K4me3 level-associated transcriptional activation of IGF2 in human nasal epithelial cells. The gene symbol is denoted at the bottom. B-D Primary human nasal epithelial cells with the SMYD3-mediated H3K4me3.^high status express high levels of IGF2, as determined by ELISA (B), immunoblotting (C) and quantitative RT‒PCR (D). E Immunoblot analysis of IGF2 protein expression in ITs from the control group and NPs from the Th2 CRSwNP and non-Th2 CRSwNP groups (n = 9). Statistical significance (B and D), one-way ANOVA;***p < 0.001;**p < 0.01; ∗ p < 0.05; ns, not significant (p > 0.05)