Fig. 5

Autocrine GM-CSF, OSM, and LIF in TF-1(R140Q) cells determined the cytokine-independent transformation of TF-1(R140Q) cells. A mRNA expression levels of GM-CSF, IL-3, IL-6, G-CSF, LIF, OSM, and IL-11 in TF-1(WT) and TF-1(R140Q) cells in the absence of GM-CSF for 24 h. ***P < 0.001, compared with TF-1(WT) cells. B Secretion of GM-CSF, OSM, and LIF from TF-1(WT) and TF-1(R140Q) cells in the absence of GM-CSF for 24 h. ***P < 0.001, compared with TF-1(WT) cells. C Effects of AGI-6780 treatment on the mRNA expression of GM-CSF, OSM, and, LIF in TF-1(R140Q) cells in the absence of GM-CSF for 24 h. ***P < 0.001 compared with the control (0 µM AGI-6780). D Effects of AGI-6780 on GM-CSF, OSM, and LIF protein secretion in TF-1(R140Q) cells in the absence of GM-CSF for 24 h. ***P < 0.001 compared with the control (0 µM of AGI-6780). The dose of “0” group represents the vehicle control