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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: IDH2/R140Q mutation confers cytokine-independent proliferation of TF-1 cells by activating constitutive STAT3/5 phosphorylation

Fig. 3

Effects of AGI-6780 on STAT3 and STAT5 activation in TF-1(R140Q) cells. A TF-1(R140Q) cells were treated with AGI-6780 for 2 days and subjected to western blotting for detection of the indicated proteins. GAPDH was used as a loading control. Ratios of the mean gray value of phospho-STAT3 (Tyr705) to the mean gray value of STAT3 (B), ratio of the mean gray value of pSTAT5 (Tyr694) to the mean gray value of STAT5 (C) and ratios in the treated group to the 0 µM of AGI-6780 group were analyzed. ***P < 0.001 compared with 0 µM of AGI-6780. D TF-1(R140Q) cells were treated with AGI-6780 for 7 days in the absence of GM-CSF, and cell viability was assayed by the MTT method. The inhibition rate was evaluated. The results are repetitive of three independent experiments with similar results, and error bars indicates standard deviations. ***P < 0.001 compared with 0 µM of AGI-6780 for 7 days. The dose of “0” group represents the vehicle control

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Cell Communication and Signaling

ISSN: 1478-811X