Fig. 2
Aberrant constitutive activation of STAT3 and STAT5 with induced phospho-STAT3(Tyr705 ) and phospho-STAT5(Tyr694 ) levels in TF-1(R140Q) cells. A TF-1(WT) and TF-1(R140Q) cells were cultured in the absence or presence of 5 ng/mL GM-CSF for 24 h and subjected to western blotting for detection of the indicated proteins. B, C Ratios of the mean gray values of the indicated phosphorylated proteins to the mean gray values of the indicated total proteins and rations of values in TF-1(WT) and TF-1(R140Q) cells in the absence or presence of GM-CSF were analyzed. Representative results of three independent experiments are shown. Error bars are standard deviations. *P < 0.05, ***P < 0.001 compared with TF-1(WT) in the absence of GM-CSF; ###P < 0.001 compared with TF-1(WT) in the presence of GM-CSF; ns indicates no significance. The dose of “0” group represents the vehicle control