Fig. 6

Viability studies of K562 cells co-cultured with MSC spheroids and treated with TKI ± BMP inhibitor. Ai Workflow schematic of pre-labelling K562 cells with the proliferation dye CTV prior to simultaneous seeding with MSCs. Aii Example microwells seeded with MSCs + K562-CTV, Day1- treatment start with the TKIs; IM (1 µM) or SC (1 µM, 2 µM), DOR (2.5 µM) and the combination TKI (1 µM) + DOR, and Day 4, viability staining with FDA and PI 72 h post treatment start. Blue = CTV, Green = FDA, Red = PI. Aiii Co-localization analyses of CTV positive and FDA/PI positive cell population were performed with the co-localization plug-in tool JACoP. Two-Way ANOVAs were performed using GraphPad Prism 8. Bi Co-culture studies in micropatterned plates were imaged after 72 h of drug treatment prior to subsequent CD235A-APC and CD73-PE and (Bii-iv) apoptosis staining. Apoptosis stains were measured by flow cytometry, expressed as mean ± SD (n = 3) and were compared to the NDC by Two-Way-ANOVAs in GraphPad Prism 8. The grade of significance is indicated by asterisks (**** p < 0.0001, *** p 0.0001 to 0.001, ** p 0.001 to 0.01, * p 0.01 to 0.05). See also Figure S7