Fig. 5

Gene expression comparison analyses of CP-CML and normal CD34⁺ cells following drug treatments. Ai + ii Fluidigm gene expression analyses of CP-CML and normal CD34⁺ cells treated with IM, SC or DOR, and the combination (IM = 1 µM, SC = 1 µM, 2 µM, 5 µM; DOR = 2.5 µM; Combo = 1 µM TKI (IM or SC) + 2.5 µM DOR) in absence or presence of BMP4 (20 ng/ml), or in co-culture with HS-5 at 72 h. Gene expression heat maps (-ddCt) of treated CP-CML and normal CD34.⁺ cells resulted in treatment clustering for culture conditions. B GSEA enrichment plots for CP-CML (i) monocultures without BMP4 and (ii) co-culture treated with SC + DOR shown together with nominal enrichment score (NES), p-value and False Discovery Rate (FDR). Positive values indicate upregulation of gene sets and negative values indicate downregulation of gene sets. See also Figure S5 and S6