Fig. 3

SC and DOR induce apoptosis and an increase in Sub G0 in CP-CML CD34⁺ cells. A Annexin V/ 7AAD apoptosis analysis of CP-CML CD34⁺ cells treated with the TKIs IM or SC and BMP receptor inhibitor DOR and the combination (IM = 1 µM, SC = 1 µM, 2 µM, 5 µM; DOR = 2.5µM; Combo = 1 µM TKI (SC or IM) + 2.5 µM DOR) in absence or presence of BMP4 (20 ng/ml) or in co-culture with HS-5 at 72 h. Aii + iii Apoptosis increases in CP-CML CD34.⁺ samples after treatment with SC and DOR in the presence and absence of BMP4. Aii-iv Comparison of mono- and co-culture conditions reveal an increase in viable cells and a decrease of cells in late apoptosis when co-cultured with HS-5. Bi + ii Cell cycle analyses shows a significant increase in the number of cells in Sub-G0 in all single and dual treatments of CP-CML samples. Biii Similar results were observed in presence of BMP4. Biv HS-5 co-cultures showed an overall protective effect with no changes observed after treatment. Data are expressed as mean ± SD (n = 3) and were compared using Two-Way-ANOVA (**** p < 0.0001, *** p 0.001 to 0.0001, ** p 0.01 to 0.001, * p 0.05 to 0.01). See also Figure S2