Fig. 1

Effects of SC on CML cell line K562. Ai-iii K562 cells were treated with different SC concentrations and metabolic activity monitored over 72 h to establish an IC₅₀. B Cells were treated with SC (250 nM, 500 nM, 1 µM, 2 µM or 5 µM) or IM (350 nM) over 72 h and viable cells counts determined by trypan blue exclusion. Ci-iii Apoptosis was measured by flow cytometry. Example gates taken from FlowJo V10 showing one measured set of the three replicate experiments at 72 h. Di-iii K562 cells were fixed after 24 h, 48 h and 72 h followed by DAPI staining to determine the percentage of cells in each stage of cell cycle. The histograms demonstrate the cell cycle distribution of each treatment arm for 24 h, 48 h and 72 h for one replicate. All data are expressed as mean ± SD (n = 3) and treatments were compared to the NDC by Two-Way-ANOVAs in GraphPad Prism 8. The grade of significance is indicated by asterisks (**** p < 0.0001, *** p 0.001 to 0.0001, ** p 0.01 to 0.001, * p 0.05 to 0.01)