Fig. 1

PKCδ, CD31, α-SMA, and CD68 staining in a femoral artery tissue from lower limb of a 60 years diabetes patient who had underwent an amputation surgery (A, B). Representative images of HE staining of vessels. C Positive co-staining of CD31/PKCδ was observed in intimal layer. CD31 shown in green, PKCδ in red, and DAPI in blue. D Positive co-staining of α-SMA/PKCδ was observed in media layer. α-SMA shown in green, PKCδ in red, and DAPI in blue. E Positive co-staining of CD68/PKCδ was observed in neointimal and adventitial layers. CD68 shown in green, PKCδ in red, and DAPI in blue. The magnification scale of HE image was 5X. Arrows show positive colocalized staining. L, lumen; M, media; N, neointima; Adv, adventitia