Fig. 6
From: Simvastatin induces pyroptosis via ROS/caspase-1/GSDMD pathway in colon cancer
Simvastatin inhibits pyroptosis and tumor growth in vivo. A Representative photographs of isolated tumors in each group. The tumor tissues from two rows were taken from sham group and simvastatin group, respectively. B The tumor growth curve of each treatment group was presented in line charts. The volume of tumors from mice of each group was measured and recorded twice a week for 4 weeks. C The weight of isolated tumors in each group was presented in bar charts. D Tumor tissues were fixed with formaldehyde and embedded by paraffin for H&E staining. E Levels of ROS in isolated tumors were detected by using DCFH-DA probe and flow cytometry at day 28 after treatment. F levels of NLRP3, ASC, pro-Caspase-1, cleaved-Caspase-1, pro-IL-1β, mature-IL-1β, IL-18, GSDMD, GSDMD-N were tested in tumors from mice of each group, with β-actin as the reference protein. *, p < 0.05