Fig. 3
From: Simvastatin induces pyroptosis via ROS/caspase-1/GSDMD pathway in colon cancer
Simvastatin increased caspase-1 dependent pyroptosis in colon cancer cells. A The viability of HCT116 and SW620 cells was detected by CCK-8 assay after exposure to different drugs. B Pyroptosis rate of different groups of cells was detected by flow cytometry analysis. C Cells expressed activated caspase-1 were labeled by immunofluorescence staining. D Levels of pro-caspase1, cleaved-caspase1, pro-IL-1β, mature-IL-1β and IL-18 were detected by western blotting analysis, with β-actin as the reference protein. *, p < 0.05