Fig. 6
PI3K/Akt and Wnt/β-catenin pathways were connected by GSK3β in HCC. A Huh7 and Hep3B cells were treated with the indicated concentrations of LY294002 for 24 h. PI3K/Akt and Wnt/β-catenin pathways associated molecules were detected using western blot analysis. B Schematic illustration of the experiments on the mice transfected with HrasG12V and miRp53 (n = 5)-injected group and HrasG12V, miRp53, and PI3KE545K-injected group (n = 5). C Body weight of each group was confirmed every week for 3 weeks. D Number of nodules in mouse liver of each group was counted. E PI3K/Akt and Wnt/β-catenin pathways associated molecules were detected using western blot analysis in mouse liver tumors. F H&E and IHC staining of p-GSK3β-Ser9 and p-GSK3β-Tyr216 in mouse liver. Data are presented as the mean ± SD and performed in triplicate independently. Statistical significance is indicated by *P < 0.05, **P < 0.01