Fig. 5
SOR + WAY treatment inhibited the PI3K/Akt and Wnt/β-catenin pathways through GSK3β activation in vivo. A Schedule for the experiments on the mice transfected with HrasG12V and shp53. Mice were divided into four groups: Control (n = 4), SOR (n = 4), WAY (n = 5), SOR + WAY (n = 5). Mice of each group were orally administered for 10 d before sacrificed. B Representative liver pictures of each group. C Number of nodules in mouse liver of each group was counted. D PI3K/Akt and Wnt/β-catenin pathways associated molecules were detected using western blot analysis in mouse liver tumors. E H&E, IF and IHC staining of DKK1, active β-catenin, p-GSK3β-Ser9 and p-GSK3β-Tyr216 in mouse liver. F IF staining of cleaved Caspase-3 and Ki-67 in mouse liver. Data are presented as the mean ± SD and performed in triplicate independently. Statistical significance is indicated by *P < 0.05, **P < 0.01, ***P < 0.001