Fig. 4

SOR + WAY treatment inhibited the PI3K/Akt and Wnt/β-catenin pathways through GSK3β activation in vitro. Huh7 and Hep3B cells were treated with IC₅₀ values of SOR and/or WAY for 24 h. A Numbers of differentially expressed genes by comparing SOR to SOR + WAY treatment in Huh7 and Hep3B cells. B PI3K/Akt and Wnt/β-catenin pathways associated molecules were detected using western blot analysis in Huh7 and Hep3B cells. C qRT-PCR analysis of PI3K/Akt and Wnt/β-catenin pathways associated genes in Huh7 and Hep3B cells. D Huh7 and Hep3B cells were treated with IC₅₀ values of SOR and/or 25 μM LY294002 for 24 h. PI3K/Akt and Wnt/β-catenin pathways were detected using western blot analysis in Huh7 and Hep3B cells. E Huh7 and Hep3B cells were treated with indicated concentrations of SOR and/or LY294002 for 10 d. Subsequently, long-term colony formation assay was confirmed. Data are presented as the mean ± SD and performed in triplicate independently. Statistical significance is indicated by *P < 0.05, **P < 0.01, ***P < 0.001