Fig. 4

The abnormality rate of MDS erythroblast nuclei in the high risk group was higher than that in the normal group and low-middle risk group. A The morphology of erythroblasts from MDS patients with different risk stratifications cultured in vitro (the black arrow indicates malformed nucleus), with scale bar = 10 μm. B Quantitative analysis of the enucleation efficiency of erythroblasts from MDS patients with different risk stratifications. Nuclear malformations include binucleation, nuclear budding, multinucleation, and megaloblastosis, 100 cells of each group from three independent experiments were used for the quantification. The results are all expressed as the mean ± SD; * indicates p < 0.05,** indicates p < 0.01,*** indicates P < 0.001