Fig. 1

The enucleation of erythroblasts in the MDS group was lower than that in the normal group. A Gating strategy for erythroblast enucleation. (A:a) All events are displayed on this plot of forward scatter area vs side scatter area, exclusion of dead cells and debris. (A:b-c) Exclusion of doublets on a plot of forward scatter area vs height and side scatter area vs height. (A:d) Singles are displayed. Hoechst negative cells are gated, these are mature red blood cells. B Flow cytometry detection of erythroblast enucleation in normal control individuals and patients with different subtypes of MDS. (B: a-d) Normal group of erythroblast enucleation. (B: e-j) Erythroblast enucleation in the MDS subtype was RA, RARS, RCMD, RAEB-1, RAEB-2 and MDS-U. C Quantitative analysis statistics of the rate of enucleation in the MDS and normal groups. D Pearson correlation analysis showed a positive correlation between enucleation and hemoglobin concentration in MDS patients (r = 0.5992, p < 0.01). The results are expressed as the mean ± SD, and * represents p < 0.05, ** represents p < 0.01, and *** represents p < 0.001