Fig. 5

IL-1β mediates PD-L1 and PD-L2 induction concomitant with IDO1 induction in lung adenocarcinoma cells. A Expression of CD274 and IDO1 mRNA in human lung adenocarcinoma. Each dot represents a lung adenocarcinoma tumor from TCGA (n = 510). B Expression of PDCD1LG2 and IDO1 mRNA in human lung adenocarcinoma. Each dot represents a lung adenocarcinoma tumor from TCGA (n = 510). C—D RT-qPCR measurement of CD274 mRNA (C) and PDCD1LG2 mRNA (D) induction in lung adenocarcinoma cells stimulated with 5 ng/mL IL-1β for 48 h. E Western blots showing PD-L1 protein (CD274 gene) and IDO1 protein induction in lung adenocarcinoma cells. F Schematic showing the experimental set-up for (G). G Western blot for IDO1 and PD-L1 protein in HCC827 cells at 24 and 48 h after 5 ng/mL IL-1β stimulation and their levels 24 and 48 h after the removal of the stimulation. GAPDH was used as the loading control. Error bars represent ± SD of 3 biological replicates: *P ≤ 0.05, ** ≤ 0.005, *** ≤ 0.0005. mRNA levels were normalized to 18s mRNA and represented as fold change over control treatment