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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: IL-1β mediates the induction of immune checkpoint regulators IDO1 and PD-L1 in lung adenocarcinoma cells

Fig. 3

IL-1β regulates IDO1 transcription by activating the NFκB pathway in lung adenocarcinoma. A H1792 and HCC827 cells transfected with 40 nM of non-targeting or RELA/p65 siRNA (Dharmacon) for 24 h followed by treatment with vehicle (ctrl) or 5 ng/mL IL-1β for 48 h and RT-qPCR for RELA mRNA was performed to validate the efficacy of knockdown. B RT-qPCR for CXCL8 mRNA indicates repression of p65 transcriptional activity in H1792 and HCC827 cells. C RT-qPCR for IDO1 mRNA levels indicates p65 regulates IDO1 transcription in H1792 and HCC827 cells. D Western blot showing levels of IDO1 and p65/RelA protein levels. E Western blot of HCC827 cells treated with 5 ng/ml IL-1β ± BAY11-7085 (p65/NFκB inhibitor) for 48 h. Error bars represent ± SD of 3 biological replicates: *P ≤ 0.05, ** ≤ 0.005, *** ≤ 0.0005. mRNA levels were normalized to 18s mRNA and represented as fold change over control treatment. GAPDH is the Western blot loading control

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Cell Communication and Signaling

ISSN: 1478-811X